Abstract： Objective To establish the HPLC fingerprints of Citri Eoxcarpium Rubrum medicinal materials,decoction pieces,standard decoction and formula granules,and analyze their correlation and difference.Methods Gradient elution was performed on a CORTECS T3(150 mm × 4.6 mm,2.7μm)column with acetonitrile(A)and 0.05％phosphoric acid(B)as the mobile phase.The flow rate was 1.1 mL·min-1,the detection wavelength was 300 nm,the column temperature was 30 ℃,and the injection volume was 10 μL.The HPLC fingerprints of 15 batches of Citri Eoxcarpium Rubrum medicinal materials,decoction pieces,standard decoction and 3 batches of formula granules were determined with the hesperidin peak as the reference peak.The Similarity Evaluation System for Chromatographic Fingerprints of Traditional Chinese Medicine(Version 2012)was used to evaluate the similarity and identify common peaks,and the cluster analysis was conducted.Results There were 12 common peaks in the HPLC fingerprints of Citri Eoxcarpium Rubrum medicinal materials,decoction pieces,standard decoction and 3 batches of formula granules,and 7 of them were identified.Peak 4 was vicenin-2,peak 6 was ferulic acid,peak 7 was narirutin,peak 8 was hesperidin,peak 10 was nobiletin,peak 11 was 3,5,6,7,8,3',4'-heptamethoxyflavone,and peak 12 was tangeretin.The similarity between each of the Citri Eoxcarpium Rubrum medicinal materials,decoction pieces,standard decoction and formula granules fingerprints and its corresponding control fingerprint was greater than 0.980.The correlation analysis showed that most common peaks were significantly correlated and the cluster analysis showed no significant difference among the medicinal materials,decoction pieces,standard decoction and formula granules.The content transfer rate from decoction pieces to formula granules was 21.7％～26.8％with an average value of 24.8％.No dispersion was found within±30％of the mean,indicating good transferability.Conclusion The main chemical components of the medicinal materials,decoction pieces,standard decoction and formula granules of Citri Eoxcarpium Rubrum are basically the same.With good correlation,the fingerprint could provide a reference for the quality control of the production of the formula granules of Citri Eoxcarpium Rubrum.