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目的:利用基因表达数据库(GEO)和Oncomine数据库筛选与肺腺癌发生发展相关的关键基因，并对其在肺腺癌患者预后中的价值进行分析。方法:利用GEO对肺腺癌表达数据集(GSE10072)进行基因分层富集分析，利用Oncomine对肺腺癌的相关研究进行荟萃分析筛选上调和下调基因。对两个结果比对筛选在两个结果中都存在的基因，利用GEPIA在线工具评价这些基因对肺腺癌患者生存期的影响。结果:基因富集分析结果显示，吸烟肺腺癌富集信号通路最多，戒烟肺腺癌富集到的通路次之，不吸烟肺腺癌富集的信号通路最少。将基因富集分析结果中前100个基因与Oncomine荟萃分析结果比较，筛选出肺腺癌中27个上调和44个下调的关键基因。进一步生存分析结果显示，13个上调和6个下调的基因可影响肺腺癌患者预后(所有Logrank P<0.05)。 结论:对不同吸烟状态肺腺癌发生发展的信号通路进行分析后，筛选出来的19个肺腺癌发生发展关键基因，可以在后续的研究中进一步评价这些标志物在肺腺癌中的诊断价值以及应用价值。

Objective:To screen the key genes involved in development of lung adenocarcinoma using gene expression omnibus (GEO) and Oncomine databases, and to evaluate the value of them in predicting prognosis of lung adenocarcinoma patients.Methods:Gene data-set enrichment analysis based on GEO was used for gene expression profile GSE10072. A meta-analysis of lung adenocarcinoma studies based on oncomine was used to screen up-regulated and down-regulated genes. The results of the two databases were compared to screen the genes presented in both results, and the GEPIA online tool was used to evaluate the effect of these genes on the survival of lung adenocarcinoma patients.Results:Results of gene set enrichment analysis showed that the most pathways enriched in current smoker lung adenocarcinoma patients, followed by quit-smoker lung adenocarcinoma patients and non-smoker lung adenocarcinoma patients. According to comparison of top 100 genes in gene data-set enrichment analysis and results of the Oncomine meta-analysis, 27 up-regulated and 44 down-regulated key genes were identified. Results of survival analysis showed that 13 up-regulated and 6 down-regulated genes might affect the overall survival of lung adenocarcinoma patients (all logrank P<0.05). Conclusions:After analyzing the signaling pathways of the occurrence and development of lung adenocarcinoma in patients with different smoking states, 19 key genes for the occurrence and development of lung adenocarcinoma are screened out, of which the diagnostic value and application value for patients with lung adenocarcinoma can be further evaluated in subsequent studies.